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Histological Correlates of Diffusion-Weighted Magnetic Resonance Microscopy in a Mouse Model of Mesial Temporal Lobe Epilepsy

Göbel-Guéniot, Katharina; Gerlach, Johannes; Kamberger, Robert; Leupold, Jochen; vElverfeldt, Dominik von; Hennig, Jürgen; Korvink, Jan G.; Haas, Carola A.; LeVan, Pierre

Mesial temporal lobe epilepsy (MTLE) is the most common type of focal epilepsy. It is frequently associated with abnormal MRI findings, which are caused by underlying cellular, structural, and chemical changes at the micro-scale. In the current study, it is investigated to which extent these alterations correspond to imaging features detected by high resolution magnetic resonance imaging in the intrahippocampal kainate mouse model of MTLE. Fixed hippocampal and whole-brain sections of mouse brain tissue from nine animals under physiological and chronically epileptic conditions were examined using structural and diffusion-weighted MRI. Microstructural details were investigated based on a direct comparison with immunohistochemical analyses of the same specimen. Within the hippocampal formation, diffusion streamlines could be visualized corresponding to dendrites of CA1 pyramidal cells and granule cells, as well as mossy fibers and Schaffer collaterals. Statistically significant changes in diffusivities, fractional anisotropy, and diffusion orientations could be detected in tissue samples from chronically epileptic animals compared to healthy controls, corresponding to microstructural alterations (degeneration of pyramidal cells, dispersion of the granule cell layer, and sprouting of mossy fibers). ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000120003
Veröffentlicht am 05.06.2020
DOI: 10.3389/fnins.2020.00543
Web of Science
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Mikrostrukturtechnik (IMT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 1662-453X
KITopen-ID: 1000120003
HGF-Programm 43.22.01 (POF III, LK 01) Functionality by Design
Erschienen in Frontiers in neuroscience
Verlag Frontiers Media
Band 14
Seiten Article no: 543
Vorab online veröffentlicht am 03.06.2020
Schlagwörter MR microscopy, HARDI, tractography, hippocampus, mesial temporal lobe epilepsy, kainate
Nachgewiesen in Web of Science
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