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Enhanced Stability of Lipid Structures by Dip-Pen Nanolithography on Block-Type MPC Copolymer

Liu, Hui-Yu; Kumar, Ravi; Takai, Madoka; Hirtz, Michael

Abstract (englisch):
Biomimetic lipid membranes on solid supports have been used in a plethora of applications, including as biosensors, in research on membrane proteins or as interfaces in cell experiments. For many of these applications, structured lipid membranes, e.g., in the form of arrays with features of different functionality, are highly desired. The stability of these features on a given substrate during storage and in incubation steps is key, while at the same time the substrate ideally should also exhibit antifouling properties. Here, we describe the highly beneficial properties of a 2-methacryloyloxyethyl phosphorylcholine (MPC) copolymer for the stability of supported lipid membrane structures generated by dip-pen nanolithography with phospholipids (L-DPN). The MPC copolymer substrates allow for more stable and higher membrane stack structures in comparison to other hydrophilic substrates, like glass or silicon oxide surfaces. The structures remain highly stable under immersion in liquid and subsequent incubation and washing steps. This allows multiplexed functionalization of lipid arrays with antibodies via microchannel cantilever spotting (µCS), without the need of orthogonal binding tags for each antibody type. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000120185
Veröffentlicht am 17.06.2020
DOI: 10.3390/molecules25122768
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Nanotechnologie (INT)
Karlsruhe Nano Micro Facility (KNMF)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 15.06.2020
Sprache Englisch
Identifikator ISSN: 1420-3049
KITopen-ID: 1000120185
HGF-Programm 43.22.03 (POF III, LK 01) Printed Materials and Systems
Weitere HGF-Programme 49.01.01 (POF III, LK 02) INT-KNMF Structuring Lab
Erschienen in Molecules
Verlag MDPI
Band 25
Heft 12
Seiten Article no: 2768
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Gefördert vom Ministerium für Wissenschaft, Forschung und Kunst Baden-Württemberg (MWK) im Rahmen des Open-Access-Förderprogramms "BW BigDIWA"
Vorab online veröffentlicht am 14.06.2020
Schlagwörter Project-ID: 2018-020-023711 (DPN)
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