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Long non-coding RNA LASSIE regulates shear stress sensing and endothelial barrier function

Stanicek, Laura; Lozano-Vidal, Noelia; Bink, Diewertje Ilse; Hooglugt, Aukie; Yao, Wenjie; Wittig, Ilka; Rijssel, Jos van; Buul, Jaap Diederik van; Bergen, Anke van; Klems, Alina 1; Ramms, Anne Sophie 1; Le Noble, Ferdinand 1; Hofmann, Patrick; Szulcek, Robert; Wang, ShengPeng; Offermanns, Stefan; Ercanoglu, Meryem Seda; Kwon, Hyouk-Bum; Stainier, Didier; ... mehr


Blood vessels are constantly exposed to shear stress, a biomechanical force generated by blood flow. Normal shear stress sensing and barrier function are crucial for vascular homeostasis and are controlled by adherens junctions (AJs). Here we show that AJs are stabilized by the shear stress-induced long non-coding RNA LASSIE (linc00520). Silencing of LASSIE in endothelial cells impairs cell survival, cell-cell contacts and cell alignment in the direction of flow. LASSIE associates with junction proteins (e.g. PECAM-1) and the intermediate filament protein nestin, as identified by RNA affinity purification. The AJs component VE-cadherin showed decreased stabilization, due to reduced interaction with nestin and the microtubule cytoskeleton in the absence of LASSIE. This study identifies LASSIE as link between nestin and VE-cadherin, and describes nestin as crucial component in the endothelial response to shear stress. Furthermore, this study indicates that LASSIE regulates barrier function by connecting AJs to the cytoskeleton.

Verlagsausgabe §
DOI: 10.5445/IR/1000120269
Veröffentlicht am 18.06.2020
DOI: 10.1038/s42003-020-0987-0
Zitationen: 27
Web of Science
Zitationen: 27
Zitationen: 34
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische und Chemische Systeme (IBCS)
Zoologisches Institut (ZOO)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 12.2020
Sprache Englisch
Identifikator ISSN: 2399-3642
KITopen-ID: 1000120269
HGF-Programm 47.01.01 (POF III, LK 01) Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Erschienen in Communications biology
Verlag Nature Research
Band 3
Heft 1
Seiten Art.Nr. 265
Vorab online veröffentlicht am 26.05.2020
Nachgewiesen in Scopus
Web of Science
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