This work investigated the effect of steel substrate topography and roughness on cathodic disbonding resistance and wet adhesion of the polyvinyl butyral/oxide/steel interface. Laser structuring was employed to pattern steel surfaces with controlled, periodic peaks of different peak-to-valley height, Rz, and geometry. Grinded smooth samples were used for reference. The in-situ scanning Kelvin probe technique was used to follow the cathodic disbonding in humid air and wet adhesion loss in inert atmosphere (3 ppm O2). Both cathodic disbonding and wet adhesion loss depended on the ability of the surface for mechanical adhesion, even when compensating for the increased effective contact area. X-ray photoelectron spectroscopy excluded the possibility for oxide chemistry effects on the delamination rate. Surfaces with features that enabled mechanical interlocking forces, had the best cathodic disbonding resistance and wet adhesion properties.