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Pcdh18a regulates endocytosis of E-cadherin during axial mesoderm development in zebrafish

Bosze, B. 1; Ono, Y.; Mattes, B.; Sinner, C. 2; Gourain, V. 1; Thumberger, T.; Tlili, S.; Wittbrodt, J.; Saunders, T. E.; Strähle, U. 1; Schug, A. 2; Scholpp, S. 1
1 Institut für Toxikologie und Genetik (ITG), Karlsruher Institut für Technologie (KIT)
2 Scientific Computing Center (SCC), Karlsruher Institut für Technologie (KIT)


The notochord defines the axial structure of all vertebrates during development. Notogenesis is a result of major cell reorganization in the mesoderm, the convergence and the extension of the axial cells. However, it is currently not fully understood how these processes act together in a coordinated way during notochord formation. The prechordal plate is an actively migrating cell population in the central mesoderm anterior to the trailing notochordal plate cells. We show that prechordal plate cells express Protocadherin 18a (Pcdh18a), a member of the cadherin superfamily. We find that Pcdh18a-mediated recycling of E-cadherin adhesion complexes transforms prechordal plate cells into a cohesive and fast migrating cell group. In turn, the prechordal plate cells subsequently instruct the trailing mesoderm. We simulated cell migration during early mesoderm formation using a lattice-based mathematical framework and predicted that the requirement for an anterior, local motile cell cluster could guide the intercalation and extension of the posterior, axial cells. Indeed, a grafting experiment validated the prediction and local Pcdh18a expression induced an ectopic prechordal plate-like cell group migrating towards the animal pole. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000120775
Veröffentlicht am 10.07.2020
DOI: 10.1007/s00418-020-01887-5
Zitationen: 7
Web of Science
Zitationen: 5
Zitationen: 8
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische und Chemische Systeme (IBCS)
Scientific Computing Center (SCC)
Universität Karlsruhe (TH) – Zentrale Einrichtungen (Zentrale Einrichtungen)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 0301-5564, 0948-6143, 1432-119X
KITopen-ID: 1000120775
HGF-Programm 47.01.01 (POF III, LK 01) Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Weitere HGF-Programme 46.11.01 (POF III, LK 01) Computational Science and Mathematical Methods
Erschienen in Histochemistry and cell biology
Verlag Springer
Band 154
Seiten 463–480
Vorab online veröffentlicht am 01.06.2020
Nachgewiesen in Web of Science
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