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Influence of wood species on toxicity of log-wood stove combustion aerosols: A parallel animal and air-liquid interface cell exposure study on spruce and pine smoke

Ihantola, T.; Di Bucchianico, S.; Happo, M.; Ihalainen, M.; Uski, O.; Bauer, S.; Kuuspalo, K.; Sippula, O.; Tissari, J.; Oeder, S.; Hartikainen, A.; Rönkkö, T. J.; Martikainen, M.-V.; Huttunen, K.; Vartiainen, P.; Suhonen, H.; Kortelainen, M.; Lamberg, H.; Leskinen, A.; Sklorz, M.; ... mehr

Abstract:
Background
Wood combustion emissions have been studied previously either by in vitro or in vivo models using collected particles, yet most studies have neglected gaseous compounds. Furthermore, a more accurate and holistic view of the toxicity of aerosols can be gained with parallel in vitro and in vivo studies using direct exposure methods. Moreover, modern exposure techniques such as air-liquid interface (ALI) exposures enable better assessment of the toxicity of the applied aerosols than, for example, the previous state-of-the-art submerged cell exposure techniques.

Methods
We used three different ALI exposure systems in parallel to study the toxicological effects of spruce and pine combustion emissions in human alveolar epithelial (A549) and murine macrophage (RAW264.7) cell lines. A whole-body mouse inhalation system was also used to expose C57BL/6 J mice to aerosol emissions. Moreover, gaseous and particulate fractions were studied separately in one of the cell exposure systems. After exposure, the cells and animals were measured for various parameters of cytotoxicity, inflammation, genotoxicity, transcriptome and proteome.
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Verlagsausgabe §
DOI: 10.5445/IR/1000121323
Veröffentlicht am 24.07.2020
Originalveröffentlichung
DOI: 10.1186/s12989-020-00355-1
Scopus
Zitationen: 5
Web of Science
Zitationen: 4
Dimensions
Zitationen: 7
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische und Chemische Systeme (IBCS)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 1743-8977
KITopen-ID: 1000121323
HGF-Programm 47.01.01 (POF III, LK 01) Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Erschienen in Particle and fibre toxicology
Verlag BioMed Central (BMC)
Band 17
Heft 1
Seiten Article: 27
Vorab online veröffentlicht am 15.06.2020
Nachgewiesen in Web of Science
Dimensions
Scopus
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