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Natural transformation of the filamentous cyanobacterium Phormidium lacuna

Nies, F. 1; Mielke, M. 1; Pochert, J. 1; Lamparter, T. 1
1 Karlsruher Institut für Technologie (KIT)


Research for biotechnological applications of cyanobacteria focuses on synthetic pathways and bioreactor design, while little effort is devoted to introduce new, promising organisms in the field. Applications are most often based on recombinant work, and the establishment of transformation can be a risky, time-consuming procedure. In this work we demonstrate the natural transformation of the filamentous cyanobacterium Phormidium lacuna and insertion of a selection marker into the genome by homologous recombination. This is the first example for natural transformation filamentous non-heterocystous cyanobacterium. We found that Phormidium lacuna is polyploid, each cell has about 20–90 chromosomes. Transformed filaments were resistant against up to 14 mg/ml of kanamycin. Formerly, natural transformation in cyanobacteria has been considered a rare and exclusive feature of a few unicellular species. Our finding suggests that natural competence is more distributed among cyanobacteria than previously thought. This is supported by bioinformatic analyses which show that all protein factors for natural transformation are present in the majority of the analyzed cyanobacteria.

Verlagsausgabe §
DOI: 10.5445/IR/1000121326
Veröffentlicht am 08.02.2021
DOI: 10.1371/journal.pone.0234440
Zitationen: 14
Web of Science
Zitationen: 13
Zitationen: 16
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 1932-6203
KITopen-ID: 1000121326
Erschienen in PLOS ONE
Verlag Public Library of Science (PLoS)
Band 15
Heft 6
Seiten Article: e0234440
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 12.06.2020
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