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On the applicability of the Tubulin-Based Polymorphism (TBP) genotyping method: a comprehensive guide illustrated through the application on different genetic resources in the legume family

Braglia, Luca; Gavazzi, Floriana; Morello, Laura; Gianì, Silvia; Nick, Peter; Breviario, Diego

Abstract:
Background
Plant discrimination is of relevance for taxonomic, evolutionary, breeding and nutritional studies. To this purpose, evidence is reported to demonstrate TBP (Tubulin-Based-Polymorphism) as a DNA-based method suitable for assessing plant diversity.
Results
Exploiting one of the most valuable features of TBP, that is the convenient and immediate application of the assay to groups of individuals that may belong to different taxa, we show that the TBP method can successfully discriminate different agricultural species and their crop wild relatives within the Papilionoideae subfamily. Detection of intraspecific variability is demonstrated by the genotyping of 27 different accessions of Phaseolus vulgaris.
Conclusions
These data illustrate TBP as a useful and versatile tool for plant genotyping. Since its potential has not yet been fully appreciated by the scientific community, we carefully report all the experimental details of a successful TBP protocol, while describing different applications, so that the method can be replicated in other laboratories.

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Verlagsausgabe §
DOI: 10.5445/IR/1000121994
Veröffentlicht am 24.07.2020
Originalveröffentlichung
DOI: 10.1186/s13007-020-00627-z
Scopus
Zitationen: 1
Web of Science
Zitationen: 1
Dimensions
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 12.2020
Sprache Englisch
Identifikator ISSN: 1746-4811
KITopen-ID: 1000121994
Erschienen in Plant methods
Verlag BioMed Central (BMC)
Band 16
Heft 1
Seiten Art. Nr.: 86
Vorab online veröffentlicht am 12.06.2020
Schlagwörter Genotyping, Legumes, β-Tubulins, Intron Length Polymorphism, TBP (Tubulin Based-Polymorphism)
Nachgewiesen in Dimensions
Web of Science
Scopus
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