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Pro-angiogenic Activity Discriminates Human Adipose-Derived Stromal Cells From Retinal Pericytes: Considerations for Cell-Based Therapy of Diabetic Retinopathy

Kremer, Heiner; Gebauer, Julian; Elvers-Hornung, Susanne; Uhlig, Stefanie; Hammes, Hans-Peter; Beltramo, Elena; Steeb, Lothar; Harmsen, Martin C.; Sticht, Carsten; Klueter, Harald; Bieback, Karen; Fiori, Agnese

Abstract:
Diabetic retinopathy (DR) is a frequent diabetes-associated complication. Pericyte dropout can cause increased vascular permeability and contribute to vascular occlusion. Adipose-derived stromal cells (ASC) have been suggested to replace pericytes and restore microvascular support as potential therapy of DR. In models of DR, ASC not only generated a cytoprotective and reparative environment by the secretion of trophic factors but also engrafted and integrated into the retina in a pericyte-like fashion. The aim of this study was to compare the pro-angiogenic features of human ASC and human retinal microvascular pericytes (HRMVPC) in vitro. The proliferation and the expression of ASC and HRMVPC markers were compared. Adhesion to high glucose-conditioned endothelial extracellular matrix, mimicking the diabetic microenvironment, was measured. The angiogenesis-promoting features of both cell types and their conditioned media on human retinal endothelial cells (EC) were assessed. To identify a molecular basis for the observed differences, gene expression profiling was performed using whole-genome microarrays, and data were validated using PCR arrays and flow cytometry. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000122035
Veröffentlicht am 24.07.2020
Cover der Publikation
Zugehörige Institution(en) am KIT Heidelberg Karlsruhe Research Partnership (HEiKA)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 2296-634X
KITopen-ID: 1000122035
Erschienen in Frontiers in cell and developmental biology
Band 8
Seiten Art. Nr.: 387
Vorab online veröffentlicht am 09.06.2020
Schlagwörter human adipose-derived stromal cells, human retinal pericytes, diabetic retinopathy, angiogenesis, vascular–endothelial growth factor, angiopoietin
Nachgewiesen in Scopus
Web of Science
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