Teaching old indicators even more tricks: binding affinity measurements with the guest-displacement assay (GDA)
Sinn, Stephan 1; Krämer, Joana
1; Biedermann, Frank 1
1 Institut für Nanotechnologie (INT), Karlsruher Institut für Technologie (KIT)
1; Biedermann, Frank 11 Institut für Nanotechnologie (INT), Karlsruher Institut für Technologie (KIT)
Abstract:
A simple change has important consequences: the guest-displacement assay (GDA) is introduced which allows for binding affinity determinations of supramolecular complexes with spectroscopically silent hosts and guests. GDA is complementary to indicator-displacement assay for affinity measurements with soluble components, but is superior for insoluble or for weakly binding guests.
| Zugehörige Institution(en) am KIT | Institut für Nanotechnologie (INT) |
| Publikationstyp | Zeitschriftenaufsatz |
| Publikationsjahr | 2020 |
| Sprache | Englisch |
| Identifikator | ISSN: 1359-7345, 1364-548X KITopen-ID: 1000122219 |
| HGF-Programm | 43.21.04 (POF III, LK 01) Molecular Engineering |
| Erschienen in | Chemical communications |
| Verlag | Royal Society of Chemistry (RSC) |
| Band | 56 |
| Heft | 49 |
| Seiten | 6620–6623 |
| Vorab online veröffentlicht am | 01.05.2020 |
| Nachgewiesen in | Scopus Dimensions Web of Science |