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Predicting the Effect of Chemical Factors on the pH of Crystallization Trials

Wilson, Julie; Ristic, Marko; Kirkwood, Jobie; Hargreaves, David; Newman, Janet

Abstract:
In macromolecular crystallization, success is often dependent on the pH of the experiment. However, little is known about the pH of reagents used, and it is generally assumed that the pH of the experiment will closely match that of any buffering chemical in the solution. We use a large dataset of experimentally measured solution pH values to show that this assumption can be very wrong and generate a model that can be used to successfully predict the overall solution pH of a crystallization experiment. Furthermore, we investigate the time dependence of the pH of some polyethylene glycol polymers widely used in protein crystallization under different storage conditions.

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Verlagsausgabe §
DOI: 10.5445/IR/1000122661
Veröffentlicht am 13.08.2020
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Anthropomatik und Robotik (IAR)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 06.2020
Sprache Englisch
Identifikator ISSN: 2589-0042
KITopen-ID: 1000122661
Erschienen in iScience
Verlag Cell Press
Band 23
Heft 6
Seiten Art.Nr. 101219
Nachgewiesen in Dimensions
Web of Science
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