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Fluorescent artificial receptor-based membrane assay (FARMA) for spatiotemporally resolved monitoring of biomembrane permeability

Biedermann, F.; Ghale, G.; Hennig, A.; Nau, W. M.

Abstract:
The spatiotemporally resolved monitoring of membrane translocation, e.g., of drugs or toxins, has been a long-standing goal. Herein, we introduce the fluorescent artificial receptor-based membrane assay (FARMA), a facile, label-free method. With FARMA, the permeation of more than hundred organic compounds (drugs, toxins, pesticides, neurotransmitters, peptides, etc.) through vesicular phospholipid bilayer membranes has been monitored in real time (µs-h time scale) and with high sensitivity (nM-µM concentration), affording permeability coefficients across an exceptionally large range from 10$^{-9}$–10$^{-3}$ cm s$^{-1}$. From a fundamental point of view, FARMA constitutes a powerful tool to assess structure-permeability relationships and to test biophysical models for membrane passage. From an applied perspective, FARMA can be extended to high-throughput screening by adaption of the microplate reader format, to spatial monitoring of membrane permeation by microscopy imaging, and to the compartmentalized monitoring of enzymatic activity.

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Verlagsausgabe §
DOI: 10.5445/IR/1000122781
Veröffentlicht am 21.08.2020
Originalveröffentlichung
DOI: 10.1038/s42003-020-1108-9
Scopus
Zitationen: 5
Web of Science
Zitationen: 5
Dimensions
Zitationen: 4
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Nanotechnologie (INT)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 15.07.2020
Sprache Englisch
Identifikator ISSN: 2399-3642
KITopen-ID: 1000122781
HGF-Programm 43.21.04 (POF III, LK 01) Molecular Engineering
Erschienen in Communications biology
Verlag Nature Research
Band 3
Heft 1
Seiten Art. Nr.: 383
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Nachgewiesen in Dimensions
Web of Science
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