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Fluorescent artificial receptor-based membrane assay (FARMA) for spatiotemporally resolved monitoring of biomembrane permeability

Biedermann, F. 1; Ghale, G.; Hennig, A.; Nau, W. M.
1 Institut für Nanotechnologie (INT), Karlsruher Institut für Technologie (KIT)

Abstract:

The spatiotemporally resolved monitoring of membrane translocation, e.g., of drugs or toxins, has been a long-standing goal. Herein, we introduce the fluorescent artificial receptor-based membrane assay (FARMA), a facile, label-free method. With FARMA, the permeation of more than hundred organic compounds (drugs, toxins, pesticides, neurotransmitters, peptides, etc.) through vesicular phospholipid bilayer membranes has been monitored in real time (µs-h time scale) and with high sensitivity (nM-µM concentration), affording permeability coefficients across an exceptionally large range from 109–103 cm s1. From a fundamental point of view, FARMA constitutes a powerful tool to assess structure-permeability relationships and to test biophysical models for membrane passage. From an applied perspective, FARMA can be extended to high-throughput screening by adaption of the microplate reader format, to spatial monitoring of membrane permeation by microscopy imaging, and to the compartmentalized monitoring of enzymatic activity.

Zugehörige Institution(en) am KIT Institut für Nanotechnologie (INT)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 15.07.2020
Sprache Englisch
Identifikator ISSN: 2399-3642
KITopen-ID: 1000122781
HGF-Programm 43.21.04 (POF III, LK 01) Molecular Engineering
Erschienen in Communications biology
Verlag Nature Research
Band 3
Heft 1
Seiten Art. Nr.: 383
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Nachgewiesen in Scopus
Dimensions
Web of Science

Verlagsausgabe §
DOI: 10.5445/IR/1000122781
Veröffentlicht am 21.08.2020
Originalveröffentlichung
DOI: 10.1038/s42003-020-1108-9
Scopus
Zitationen: 38
Web of Science
Zitationen: 36
Dimensions
Zitationen: 42
Seitenaufrufe: 146
seit 22.08.2020
Downloads: 132
seit 22.08.2020
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