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Improving quality in nanoparticle-induced cytotoxicity testing by a tiered inter-laboratory comparison study

Nelissen, I.; Haase, A.; Anguissola, S.; Rocks, L.; Jacobs, A.; Willems, H.; Riebeling, C.; Luch, A.; Piret, J.-P.; Toussaint, O.; Trouiller, B.; Lacroix, G.; Gutleb, A. C.; Contal, S.; Diabaté, S. 1; Weiss, C. 1; Lozano-Fernández, T.; González-Fernández, Á.; Dusinska, M.; ... mehr


The quality and relevance of nanosafety studies constitute major challenges to ensure their key role as a supporting tool in sustainable innovation, and subsequent competitive economic advantage. However, the number of apparently contradictory and inconclusive research results has increased in the past few years, indicating the need to introduce harmonized protocols and good practices in the nanosafety research community. Therefore, we aimed to evaluate if best-practice training and inter-laboratory comparison (ILC) of performance of the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay for the cytotoxicity assessment of nanomaterials among 15 European laboratories can improve quality in nanosafety testing. We used two well-described model nanoparticles, 40-nm carboxylated polystyrene (PS-COOH) and 50-nm amino-modified polystyrene (PS-NH2). We followed a tiered approach using well-developed standard operating procedures (SOPs) and sharing the same cells, serum and nanoparticles. We started with determination of the cell growth rate (tier 1), followed by a method transfer phase, in which all laboratories performed the first ILC on the MTS assay (tier 2). ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000122963
Veröffentlicht am 08.09.2020
DOI: 10.3390/nano10081430
Zitationen: 9
Zitationen: 10
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 2079-4991
KITopen-ID: 1000122963
HGF-Programm 47.01.01 (POF III, LK 01) Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Erschienen in Nanomaterials
Verlag MDPI
Band 10
Heft 8
Seiten Art.Nr. 1430
Vorab online veröffentlicht am 22.07.2020
Schlagwörter nanosafety; cytotoxicity; inter-laboratory comparison; best practice; training
Nachgewiesen in Dimensions
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