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Controlling Fibronectin Fibrillogenesis Using Visible Light

Gudzenko, T.; Franz, C. M.

Abstract:
We previously developed a surface-assisted assay to image early steps of cell-induced plasma fibronectin (FN) fibrillogenesis by timelapse atomic force microscopy (AFM). Unexpectedly, complementary attempts to visualize FN fibrillogenesis using fluorescently labeled FN (Alexa Fluor 488 or 568) and live-cell light microscopy initially failed consistently. Further analysis revealed that fibrillar remodeling was inhibited efficiently in the focal area illuminated during fluorescence imaging, but progressed normally elsewhere on the substrate, suggesting photo sensitivity of the FN fibrillogenesis process. In agreement, active cell-driven fibrillar extension of FN could be stopped by transient illumination with visible light during AFM timelapse scanning. Phototoxic effects on the cells could be ruled out, because pre-illuminating the FN layer before cell seeding also blocked subsequent fibrillar formation. Varying the illumination wavelength range between 400 and 640 nm revealed strong inhibition across the visible spectrum up to 560 nm, and a decreasing inhibitory effect at longer wavelengths. The photo effect also affected unlabeled FN, but was enhanced by fluorophore labeling of FN. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000122969
Veröffentlicht am 08.09.2020
Cover der Publikation
Zugehörige Institution(en) am KIT Center for Functional Nanostructures (CFN)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 2296-889X
KITopen-ID: 1000122969
Erschienen in Frontiers in molecular biosciences
Band 7
Seiten Art.Nr. 149
Nachgewiesen in Scopus
Web of Science
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