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Pulsed Electric Field Treatment Promotes Lipid Extraction on Fresh Oleaginous Yeast Saitozyma podzolica DSM 27192

Gorte, Olga; Nazarova, Natalja; Papachristou, Ioannis; Wüstner, Rüdiger; Leber, Klaus; Syldatk, Christoph; Ochsenreither, Katrin; Frey, Wolfgang; Silve, Aude

Abstract (englisch):
This study reports on the use of pulsed electric field (PEF) as a pre-treatment step to enhance lipid extraction yield using extraction with ethanol-hexane blend on fresh oleaginous yeast Saitozyma podzolica. The yeasts were cultivated on nitrogen-depleted condition and had a lipid content of 26.4 ± 4.6% of dry weight. PEF-treatment was applied on the yeast suspension either directly after harvesting (unwashed route) or after a washing step (washed route) which induced a reduction of conductivity by a factor eight. In both cases, cell concentration was 20 g of biomass per liter of suspension. In the unwashed route, the lipid extraction efficiency increased from 7% (untreated) to 54% thanks to PEF-treatment. In case an additional washing step was added after PEF-treatment, up to 81% of the lipid content could be recovered. The washed route was even more efficient since lipid extraction yields increased from 26% (untreated) to 99% of total lipid. The energy input for the PEF-treatment never exceeded 150 kJ per liter of initial suspension. The best lipid recovery scenario was obtained using pulses of 1 μs, an electric field of 40 kV/cm and it required slightly less than 11 MJ/kgLIPID. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000123546
Veröffentlicht am 11.09.2020
Originalveröffentlichung
DOI: 10.3389/fbioe.2020.575379
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Institut für Hochleistungsimpuls- und Mikrowellentechnik (IHM)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 2296-4185
KITopen-ID: 1000123546
Erschienen in Frontiers in Bioengineering and Biotechnology
Band 8
Seiten Article 575379
Vorab online veröffentlicht am 11.09.2020
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