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Microbial single-cell omics: the crux of the matter

Kaster, A.-K. 1,2; Sobol, M. S. 1,2
1 Institut für Angewandte Biowissenschaften (IAB), Karlsruher Institut für Technologie (KIT)
2 Institut für Biologische Grenzflächen (IBG), Karlsruher Institut für Technologie (KIT)


Single-cell genomics and transcriptomics can provide reliable context for assembled genome fragments and gene expression activity on the level of individual prokaryotic genomes. These methods are rapidly emerging as an essential complement to cultivation-based, metagenomics, metatranscriptomics, and microbial community-focused research approaches by allowing direct access to information from individual microorganisms, even from deep-branching phylogenetic groups that currently lack cultured representatives. Their integration and binning with environmental ‘omics data already provides unprecedented insights into microbial diversity and metabolic potential, enabling us to provide information on individual organisms and the structure and dynamics of natural microbial populations in complex environments. This review highlights the pitfalls and recent advances in the field of single-cell omics and its importance in microbiological and biotechnological studies.

Verlagsausgabe §
DOI: 10.5445/IR/1000123607
Veröffentlicht am 27.09.2020
DOI: 10.1007/s00253-020-10844-0
Zitationen: 30
Web of Science
Zitationen: 27
Zitationen: 36
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Biowissenschaften (IAB)
Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 10.2020
Sprache Englisch
Identifikator ISSN: 0171-1741, 0175-7598, 0340-2118, 1432-0614
KITopen-ID: 1000123607
HGF-Programm 47.02.05 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IBG-5
Erschienen in Applied microbiology and biotechnology
Verlag Springer
Band 104
Heft 19
Seiten 8209-8220
Vorab online veröffentlicht am 26.08.2020
Schlagwörter Targeted cell sorting, Amplification, Bioinformatics, Bacteria, Archaea
Nachgewiesen in Scopus
Web of Science
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