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Analysis of Structural and Functional Differences of Glucans Produced by the Natively Released Dextransucrase of Liquorilactobacillus hordei TMW 1.1822

Schmid, J.; Wefers, D.; Vogel, R. F.; Jakob, F.

Abstract:
The properties of the glucopolymer dextran are versatile and linked to its molecular size, structure, branching, and secondary structure. However, suited strategies to control and exploit the variable structures of dextrans are scarce. The aim of this study was to delineate structural and functional differences of dextrans, which were produced in buffers at different conditions using the native dextransucrase released by Liquorilactobacillus (L.) hordei TMW 1.1822. Rheological measurements revealed that dextran produced at pH 4.0 (MW = 1.1 * 10$^{8}$ Da) exhibited the properties of a viscoelastic fluid up to concentrations of 10% (w/v). By contrast, dextran produced at pH 5.5 (MW = 1.86 * 10$^{8}$ Da) was gel-forming already at 7.5% (w/v). As both dextrans exhibited comparable molecular structures, the molecular weight primarily influenced their rheological properties. The addition of maltose to the production assays caused the formation of the trisaccharide panose instead of dextran. Moreover, pre-cultures of L. hordei TMW 1.1822 grown without sucrose were substantial for recovery of higher dextran yields, since the cells stored the constitutively expressed dextransucrase intracellularly, until sucrose became available. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000123614
Veröffentlicht am 29.09.2020
Originalveröffentlichung
DOI: 10.1007/s12010-020-03407-6
Scopus
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Biowissenschaften (IAB)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 0146-0641, 0273-2289, 1559-0291, 1599-0291, 2168-8109
KITopen-ID: 1000123614
Erschienen in Applied biochemistry and biotechnology
Vorab online veröffentlicht am 21.08.2020
Schlagwörter Lactic acid bacteria, Dextransucrase, Dextran, Molecularweight, Rheology, Panose
Nachgewiesen in Scopus
Web of Science
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