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Actinomadura rubteroloni sp. nov. and Actinomadura macrotermitis sp. nov., isolated from the gut of the fungus growing-termite Macrotermes natalensis

Benndorf, René; Martin, Karin; Küfner, Michelle; de Beer, Z. Wilhelm; Vollmers, John; Kaster, Anne-Kristin; Beemelmanns, Christine

Abstract:
The taxonomic positions of two novel aerobic, Gram-positive actinobacteria, designated strains RB29$^{T}$ and RB68T, were determined using a polyphasic approach. Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbours of RB29$^{T}$ were identified as Actinomadura rayongensis DSM 102126$^{T}$ (99.2 % similarity) and Actinomadura atramentaria DSM 43919$^{T}$ (98.7 %), and for strain RB68$^{T}$ was Actinomadura hibisca DSM 44148$^{T}$ (98.3 %). Digital DNA–DNA hybridization (dDDH) between RB29$^{T}$ and its closest phylogenetic neighbours, A. rayongensis DSM 102126$^{T}$ and A. atramentaria DSM 43919$^{T}$, resulted in similarity values of 53.2 % (50.6–55.9 %) and 26.4 % (24.1–28.9 %), respectively. Additionally, the average nucleotide identity (ANI) was 93.2 % (94.0 %) for A. rayongensis DSM 102126$^{T}$ and 82.3 % (78.9 %) for A. atramentaria DSM 43919$^{T}$. dDDH analysis between strain RB68$^{T}$ and A. hibisca DSM 44148$^{T}$ gave a similarity value of 24.5 % (22.2–27.0 %). Both strains, RB29$^{T}$ and RB68$^{T}$, revealed morphological characteristics and chemotaxonomic features typical for the genus Actinomadura, such as the presence of meso-diaminopimelic acid in the cell wall, galactose and glucose as major sugar components within whole-cell hydrolysates and the absence of mycolic acids. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000124548
Veröffentlicht am 13.10.2020
Originalveröffentlichung
DOI: 10.1099/ijsem.0.004403
Scopus
Zitationen: 3
Dimensions
Zitationen: 4
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 1466-5026, 1466-5034
KITopen-ID: 1000124548
HGF-Programm 47.02.05 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IBG-5
Erschienen in International journal of systematic and evolutionary microbiology
Verlag Elsevier
Band 70
Heft 10
Seiten 5255-5262
Vorab online veröffentlicht am 26.08.2020
Schlagwörter Actinomadura , Macrotermes natalensis , new natural products, termite gut
Nachgewiesen in Scopus
Web of Science
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