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Nanosecond pulsed electric fields modulate the expression of the astaxanthin biosynthesis genes psy, crtR-b and bkt 1 in Haematococcus pluvialis

Bai, Fan 1; Gusbeth, Christian 2; Frey, Wolfgang 2; Nick, Peter 1
1 Karlsruher Institut für Technologie (KIT)
2 Institut für Hochleistungsimpuls- und Mikrowellentechnik (IHM), Karlsruher Institut für Technologie (KIT)

Abstract (englisch):

Nanosecond pulsed electric fields (nsPEFs) have been extensively studied with respect to cellular responses. Whether nsPEFs can regulate gene expression and to modulate the synthesis of valuable compounds, has so far been only tested in the context of apoptosis in cancer cells. We used the unicellular algae Haematococcus pluvialis as system to test, whether nsPEFs could alter gene expression and to promote the biosynthesis of astaxanthin. We find that nsPEFs induce a mild, but significant increase of mortality up to about 20%, accompanied by a moderate increase of astaxanthin accumulation. Steady-state transcript levels of three key genes psy, crtR-b and bkt 1 were seen to increase with a maximum at 3 d after PEF treatment at 50 ns. Pulsing at 25 ns reduce the transcripts of psy, crtR-b from around day 2 after the pulse, while those of bkt 1 remain unchanged. By blocking the membrane-located NADPH oxidase RboH, diphenylene iodonium by itself increased both, the levels of astaxanthin and transcripts of all three biosynthetic genes, and this increase was added up to that produced by nsPEFs. Artificial calcium influx by an ionophore did not induce major changes in the accumulation of astaxanthin, nor in the transcript levels, but amplified the response of crtR-b to nsPEFs at 25 ns, while decreased in 50 ns treatment. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000124897
Veröffentlicht am 20.10.2020
DOI: 10.1038/s41598-020-72479-5
Zitationen: 9
Web of Science
Zitationen: 6
Zitationen: 9
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut (BOTANIK)
Institut für Hochleistungsimpuls- und Mikrowellentechnik (IHM)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 12.2020
Sprache Englisch
Identifikator ISSN: 2045-2322
KITopen-ID: 1000124897
HGF-Programm 35.13.01 (POF III, LK 01) Biomassen und Vorbehandlung
Erschienen in Scientific reports
Verlag Nature Research
Band 10
Heft 1
Seiten Article no: 15508
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 23.09.2020
Nachgewiesen in Dimensions
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