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Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering

Schmidt, Carla; Fransz, Paul; Rönspies, Michelle; Dreissig, Steven; Fuchs, Jörg; Heckmann, Stefan; Houben, Andreas; Puchta, Holger

Abstract:
Chromosomal inversions are recurrent rearrangements that occur between different plant isolates or cultivars. Such inversions may underlie reproductive isolation in evolution and represent a major obstacle for classical breeding as no crossovers can be observed between inverted sequences on homologous chromosomes. The heterochromatic knob (hk4S) on chromosome 4 is the most well-known inversion of Arabidopsis. If a knob carrying accession such as Col-0 is crossed with a knob-less accession such as Ler-1, crossovers cannot be recovered within the inverted region. Our work shows that by egg-cell specific expression of the Cas9 nuclease from Staphylococcus aureus, a targeted reversal of the 1.1 Mb long hk4S-inversion can be achieved. By crossing Col-0 harbouring the rearranged chromosome 4 with Ler-1, meiotic crossovers can be restored into a region with previously no detectable genetic exchange. The strategy of somatic chromosome engineering for breaking genetic linkage has huge potential for application in plant breeding.

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Verlagsausgabe §
DOI: 10.5445/IR/1000125418
Veröffentlicht am 28.10.2020
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 12.2020
Sprache Englisch
Identifikator ISSN: 2041-1723
KITopen-ID: 1000125418
Erschienen in Nature Communications
Band 11
Heft 1
Seiten Art. Nr.: 4418
Projektinformation CRISBREED (EU, H2020, 741306)
Vorab online veröffentlicht am 04.09.2020
Nachgewiesen in Scopus
Web of Science
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