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A combined high-throughput and high-content platform for unified on-chip synthesis, characterization and biological screening

Benz, Maximilian; Asperger, Arndt; Hamester, Meike; Welle, Alexander; Heissler, Stefan; Levkin, Pavel A.

Abstract:
Acceleration and unification of drug discovery is important to reduce the effort and cost of new drug development. Diverse chemical and biological conditions, specialized infrastructure and incompatibility between existing analytical methods with high-throughput, nanoliter scale chemistry make the whole drug discovery process lengthy and expensive. Here, we demonstrate a chemBIOS platform combining on-chip chemical synthesis, characterization and biological screening. We developed a dendrimer-based surface patterning that enables the generation of high-density nanodroplet arrays for both organic and aqueous liquids. Each droplet (among > 50,000 droplets per plate) functions as an individual, spatially separated nanovessel, that can be used for solution-based synthesis or analytical assays. An additional indium-tin oxide coating enables ultra-fast on-chip detection down to the attomole per droplet by matrix-assisted laser desorption/ionization mass spectrometry. The excellent optical properties of the chemBIOS platform allow for on-chip characterization and in-situ reaction monitoring in the ultraviolet, visible (on-chip UV-Vis spectroscopy and optical microscopy) and infrared (on-chip IR spectroscopy) regions. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000125556
Veröffentlicht am 29.01.2021
Originalveröffentlichung
DOI: 10.1038/s41467-020-19040-0
Scopus
Zitationen: 8
Cover der Publikation
Zugehörige Institution(en) am KIT Karlsruhe Nano Micro Facility (KNMF)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 12.2020
Sprache Englisch
Identifikator ISSN: 2041-1723
KITopen-ID: 1000125556
Erschienen in Nature Communications
Verlag Nature Research
Band 11
Heft 1
Seiten Art.Nr. 5391
Vorab online veröffentlicht am 26.10.2020
Schlagwörter ToF-SIMS 2019-023-027909
Nachgewiesen in PubMed
Scopus
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