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Optimization of carbon source efficiency for lipid production with the oleaginous yeast Saitozyma podzolica DSM 27192 applying automated continuous feeding

Gorte, Olga; Kugel, Michaela; Ochsenreither, Katrin

Abstract (englisch):
Background: Biotechnologically produced microbial lipids are of interest as potential alternatives for crude and plant oils. Their lipid profile is similar to plant oils and can therefore be a substitute for the production of biofuels, additives for food and cosmetics industry as well as building blocks for oleochemicals. Commercial microbial lipids production, however, is still not profitable and research on process optimization and cost reduction is required. This study reports on the process optimization using glucose or xylose with the unconventional oleaginous yeast Saitozyma podzolica DSM 27192 aiming to reduce the applied carbon source amount without sacrificing lipid productivity. Results: By optimizing the process parameters temperature and pH, lipid productivity was enhanced by 40%. Thereupon, by establishing a two-phase strategy with an initial batch phase and a subsequent fed-batch phase for lipid production in which a constant sugar concentration of about 10 g/L was maintained, resulted in saving of ~ 41% of total glucose and ~ 26% of total xylose. By performing the automated continuous sugar feed the total sugar uptake was improved to ~ 91% for glucose and ~ 92% for xylose and thus, prevented waste of unused carbon source in the cultivation medium. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000125617
Veröffentlicht am 04.11.2020
DOI: 10.1186/s13068-020-01824-7
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 12.2020
Sprache Englisch
Identifikator ISSN: 1754-6834
KITopen-ID: 1000125617
Erschienen in Biotechnology for biofuels
Band 13
Heft 1
Seiten 181
Vorab online veröffentlicht am 02.11.2020
Schlagwörter Microbial lipids, Oleaginous yeasts, Saitozyma podzolica DSM 27192, Process optimization, Feeding strategy, Continuous feed, Glucose, Xylose, Gluconic acid, Xylonic acid
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