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Antimicrobial peptide-modified silver nanoparticles for enhancing the antibacterial efficacy

Li, W.; Li, Y. ORCID iD icon; Sun, P. 1; Zhang, N.; Zhao, Y.; Qin, S.; Zhao, Y.
1 Institut für Biologische Grenzflächen (IBG), Karlsruher Institut für Technologie (KIT)


Antibiotic-resistant bacteria are becoming a serious threat to public health worldwide. To address this problem, we have developed multifunctional peptide (MFP)-coated silver nanoparticles (MFP@AgNPs) for antibacterial studies. MFPs, which can physically adsorb to AgNPs via electrostatic interactions are comprised of a matrix metalloproteinase (MMP) cleavable sequence (PVGLIG), an antimicrobial peptide (tachyplesin-1), and a target peptide (PGP-PEG). The resulting MFP@AgNPs were characterized by various technologies, including UV-vis spectrophotometry, zeta potential analyzer, circular dichroism (CD) spectroscopy, attenuated total reflection-Fourier-transform infrared spectroscopy (ATR-FTIR), and transmission electron microscopy (TEM). The MIC and MBC were investigated against both Gram-positive bacteria and Gram-negative bacteria. The antibacterial activity in vivo was evaluated on MDR-AB (multidrug-resistant Acinetobacter baumannii) infected mice. We found that MFP@AgNPs exhibited antibacterial activity against both Gram-positive bacteria and Gram-negative bacteria. Compared to bare AgNPs, MFP@AgNPs-1 killed MDR-AB faster and more efficiently. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000126504
Veröffentlicht am 22.11.2020
DOI: 10.1039/d0ra05640e
Zitationen: 24
Web of Science
Zitationen: 21
Zitationen: 26
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 2046-2069
KITopen-ID: 1000126504
HGF-Programm 47.02.01 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IBG-1
Erschienen in RSC Advances
Verlag Royal Society of Chemistry (RSC)
Band 10
Heft 64
Seiten 38746-38754
Vorab online veröffentlicht am 22.10.2020
Nachgewiesen in Web of Science
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