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Genetically encoded thiol redox-sensors in the zebrafish model: Lessons for embryonic development and regeneration

Breus, Oksana; Dickmeis, Thomas

Abstract:
Important roles for reactive oxygen species (ROS) and redox signaling in embryonic development and regenerative processes are increasingly recognized. However, it is difficult to obtain information on spatiotemporal dynamics of ROS production and signaling in vivo. The zebrafish is an excellent model for in vivo bioimaging and possesses a remarkable regenerative capacity upon tissue injury. Here, we review data obtained in this model system with genetically encoded redox-sensors targeting H$_{2}$O$_{2}$ and glutathione redox potential. We describe how such observations have prompted insight into regulation and downstream effects of redox alterations during tissue differentiation, morphogenesis and regeneration. We also discuss the properties of the different sensors and their consequences for the interpretation of in vivo imaging results. Finally, we highlight open questions and additional research fields that may benefit from further application of such sensor systems in zebrafish models of development, regeneration and disease.

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Originalveröffentlichung
DOI: 10.1515/hsz-2020-0269
Zugehörige Institution(en) am KIT Institut für Biologische und Chemische Systeme (IBCS)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 1431-6730, 1437-4315
KITopen-ID: 1000126950
HGF-Programm 47.01.01 (POF III, LK 01) Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Erschienen in Biological chemistry
Verlag De Gruyter
Vorab online veröffentlicht am 14.10.2020
Schlagwörter embryo, glutathione, hydrogen peroxide, imaging, regeneration, zebrafish.
Nachgewiesen in Scopus
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