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Microfluidic Impedance Biosensor Chip with DNA-Based Self-Assembled Monolayers for Label-Free Detection of Cardiac Biomarker Troponin I

Alsabbagh, Khaled; Hornung, Tim; Voigt, Achim; Sadir, Sahba; Rajabi, Taleieh; Länge, Kerstin

Abstract:
A microfluidic chip for electrochemical impedance spectroscopy (EIS) is presented as biosensor for the detection of cardiac troponin I (cTnI), which is one of the most specific diagnostic serum biomarkers for myocardial infarction. Impedimetric biosensors enable the detection of a variety of analytes, including small molecules, proteins, and cells. As analyte detection is direct and label-free, they allow fast detection of biomarkers, which is essential in the diagnosis of cardiac infarctions to promote a positive outcome. The EIS chip presented here consists of a microscope glass slide serving as base plate, sputtered electrodes, and a polydimethylsiloxane (PDMS) microchannel. The electrode design mainly consists of a working electrode and a counter electrode made of gold. A silver reference electrode can be included, if required. Protocols for electrode functionalization were developed considering a low initial impedance in addition to analyte-specific binding by corresponding antibodies and reduction of non-specific protein adsorption to prevent false-positive signals. Reagents tested for self-assembled monolayers (SAM) on gold electrodes included hydrocarbons with thiol groups and thiolated oligonucleotides. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000126979
Veröffentlicht am 18.11.2021
Originalveröffentlichung
DOI: 10.3390/IECB2020-07033
Dimensions
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Mikrostrukturtechnik (IMT)
Institut für Mikroverfahrenstechnik (IMVT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 2504-3900
KITopen-ID: 1000126979
HGF-Programm 47.02.07 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IMT
Erschienen in Proceedings
Verlag MDPI
Band 60
Heft 1
Seiten 38
Bemerkung zur Veröffentlichung Proceedings / MDPI AG
Vorab online veröffentlicht am 02.11.2020
Schlagwörter biosensor; cardiac troponin I; electrochemical impedance spectroscopy; microfluidic chip
Nachgewiesen in Dimensions
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