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Heterogeneity of the osteocyte lacuno-canalicular network architecture and material characteristics across different tissue types in healing bone

Schemenz, Victoria; Gjardy, André; Chamasemani, Fereshteh F.; Roschger, Andreas; Roschger, Paul; Zaslansky, Paul; Helfen, Lukas; Burghammer, Manfred; Fratzl, Peter; Weinkamer, Richard; Brunner, Roland; Willie, Bettina M.; Wagermaier, Wolfgang

Abstract:
Various tissue types, including fibrous connective tissue, bone marrow, cartilage, woven and lamellar bone, coexist in healing bone. Similar to most bone tissue type, healing bone contains a lacuno-canalicular network (LCN) housing osteocytes. These cells are known to orchestrate bone remodeling in healthy bone by sensing mechanical strains and translating them into biochemical signals. The structure of the LCN is hypothesized to influence mineralization processes. Hence, the aim of the present study was to visualize and match spatial variations in the LCN topology with mineral characteristics, within and at the interfaces of the different tissue types that comprise healing bone. We applied a correlative multi-method approach to visualize the LCN architecture and quantify mineral particle size and orientation within healing femoral bone in a mouse osteotomy model (26 weeks old C57BL/6 mice). This approach revealed structural differences across several length scales during endochondral ossification within the following regions: calcified cartilage, bony callus, cortical bone and a transition zone between the cortical and callus region analyzed 21 days after the osteotomy. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000127620
Veröffentlicht am 12.12.2020
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Photonenforschung und Synchrotronstrahlung (IPS)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 11.2020
Sprache Englisch
Identifikator ISSN: 1047-8477
KITopen-ID: 1000127620
HGF-Programm 56.03.30 (POF III, LK 01) Soft Matter, Health and Life Sciences
Erschienen in Journal of structural biology
Verlag Elsevier
Band 212
Heft 2
Seiten Art. Nr.: 107616
Schlagwörter Endochondral ossification, Lacuno-canalicular network, qBEI, CLSM, SAXS, μCT
Nachgewiesen in Scopus
Web of Science
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