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$^{19}$F NMR of biomembranes

Grage, Stephan L.; Afonin, Sergiy; Ulrich, Anne S.

Abstract:
In this chapter we give an overview of the use of 19F NMR for the study of biomembranes. Due to its unique sensitivity and small size, fluorine may be considered to bridge the gap between NMR on the one hand, and ESR and fluorescence on the other hand. Conventional NMR isotope labels do not alter the studied molecules but possess only low sensitivity, while ESR and fluorescence labels provide higher sensitivity but also represent a larger modification of the system of interest. The high sensitivity to its environment, large anisotropies and strong dipolar couplings render 19 F a useful NMR label to probe structure and dynamics in biomembranes, and to study intermolecular interactions. Fluorine has been introduced into numerous constituents of biomembranes, such as lipids, sterols as well as membrane associated peptides and integral membrane proteins. In particular, a growing set of fluorine-labeled amino acids has allowed one to address selected structural information in membrane proteins and peptides. The large anisotropy of 19F NMR interactions in the solid-state has been used to measure molecular orientations with respect to the membrane normal, allowing characterization, for example, of the function of membrane-active peptides. ... mehr



Originalveröffentlichung
DOI: 10.1088/978-0-7503-2532-5ch6
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Institut für Organische Chemie (IOC)
Programm BioGrenzflächen (BIFTM)
Publikationstyp Buchaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISBN: 978-0-7503-2530-1
KITopen-ID: 1000127950
HGF-Programm 47.02.02 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IBG-2
Erschienen in Solid-State NMR. Applications in biomembrane structure. Ed.: F. Separovic
Verlag IOP Publishing
Seiten 6.1 - 6.43
Vorab online veröffentlicht am 03.12.2020
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