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Air–Liquid Interface Exposure of Lung Epithelial Cells to Low Doses of Nanoparticles to Assess Pulmonary Adverse Effects

Diabaté, Silvia 1; Armand, Lucie; Murugadoss, Sivakumar 1; Dilger, Marco 1; Fritsch-Decker, Susanne 1; Schlager, Christoph 2; Béal, David; Arnal, Marie-Edith; Biola-Clier, Mathilde; Ambrose, Selina; Mülhopt, Sonja ORCID iD icon 2; Paur, Hanns-Rudolf 2; Lynch, Iseult; Valsami-Jones, Eugenia; Carriere, Marie; Weiss, Carsten 1
1 Institut für Technik der Informationsverarbeitung (ITIV), Karlsruher Institut für Technologie (KIT)
2 Institut für Technische Chemie (ITC), Karlsruher Institut für Technologie (KIT)

Abstract:
Reliable and predictive in vitro assays for hazard assessments of manufactured nanomaterials (MNMs) are still limited. Specifically, exposure systems which more realistically recapitulate the physiological conditions in the lung are needed to predict pulmonary toxicity. To this end, air-liquid interface (ALI) systems have been developed in recent years which might be better suited than conventional submerged exposure assays. However, there is still a need for rigorous side-by-side comparisons of the results obtained with the two different exposure methods considering numerous parameters, such as different MNMs, cell culture models and read outs. In this study, human A549 lung epithelial cells and differentiated THP-1 macrophages were exposed under submerged conditions to two abundant types of MNMs i.e., ceria and titania nanoparticles (NPs). Membrane integrity, metabolic activity as well as pro-inflammatory responses were recorded. For comparison, A549 monocultures were also exposed at the ALI to the same MNMs. In the case of titania NPs, genotoxicity was also investigated. In general, cells were more sensitive at the ALI compared to under classical submerged conditions. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000128279
Veröffentlicht am 11.01.2021
Originalveröffentlichung
DOI: 10.3390/nano11010065
Scopus
Zitationen: 13
Web of Science
Zitationen: 12
Dimensions
Zitationen: 16
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische und Chemische Systeme (IBCS)
Institut für Technische Chemie (ITC)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 2079-4991
KITopen-ID: 1000128279
HGF-Programm 47.01.01 (POF III, LK 01) Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Weitere HGF-Programme 47.14.02 (POF IV, LK 01) Information Storage and Processing in the Cell Nucleus
Erschienen in Nanomaterials
Verlag MDPI
Band 11
Heft 1
Seiten Art. Nr.: 65
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 29.12.2020
Schlagwörter cerium dioxide; zirconium-doping; titanium dioxide; nanotoxicology; alternative methods
Nachgewiesen in Dimensions
Scopus
Web of Science
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