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Hypotonic osmotic shock treatment to enhance lipid and protein recoveries from concentrated saltwater Nannochloropsis slurries

Halim, Ronald; Papachristou, Ioannis; Kubisch, Christin; Nazarova, Natalja; Wüstner, Rüdiger; Steinbach, David; Chen, George Q.; Deng, Huining; Frey, Wolfgang; Posten, Clemens; Silve, Aude

Abstract (englisch):
This study reports the use of hypotonic osmotic shock as a treatment step to enhance the recoveries of biofuel-convertible lipids and proteins from lipid-rich saltwater Nannochloropsis gaditana (N. gaditana) slurries (biomass content = ~140 mg biomass / g slurry, total lipid content = ~600 mg lipid /g biomass). The osmotic shock was induced through repeated washing of microalgal slurries with multiple batches of fresh water. Subjecting the slurries to 2 stages of freshwater washing resulted in a measurable damage to cell membranes (the uptake of membrane permeability marker increased by 6 folds), a partial loss of cell viability (only 64% of available cells were recoverable), and a minor release of free protein (~2 wt% of available protein) from the biomass into the interstitial space of the slurries. Hypotonic osmotic shock was revealed to be ineffective in rupturing N. gaditana slurries (only 13 ± 9% of available cells were ruptured after 2-stage washing) and, as such, had a limited prospect as a stand-alone cell disruption technology for the saltwater strain. The washing treatment, however, was found to be able to weaken the structural integrity of N. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000128848
Veröffentlicht am 22.01.2021
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Hochleistungsimpuls- und Mikrowellentechnik (IHM)
Institut für Katalyseforschung und -technologie (IKFT)
Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 0016-2361
KITopen-ID: 1000128848
HGF-Programm 35.13.01 (POF III, LK 01) Biomassen und Vorbehandlung
Erschienen in Fuel
Verlag Elsevier
Band 287
Seiten Art.-Nr.: 119442
Vorab online veröffentlicht am 25.11.2020
Nachgewiesen in Scopus
Web of Science
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