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The Temperature-Regulation of Pseudomonas aeruginosa cmaX-cfrX-cmpX Operon Reveals an Intriguing Molecular Network Involving the Sigma Factors AlgU and SigX

Bouffartigues, Emeline; Si Hadj Mohand, Ishac; Maillot, Olivier; Tortuel, Damien; Omnes, Jordane; David, Audrey; Tahrioui, Ali; Duchesne, Rachel; Azuama, Cecil Onyedikachi; Nusser, Michael; Brenner-Weiss, Gerald; Bazire, Alexis; Connil, Nathalie; Orange, Nicole; Feuilloley, Marc G. J.; Lesouhaitier, Olivier; Dufour, Alain; Cornelis, Pierre; Chevalier, Sylvie

Abstract:
Pseudomonas aeruginosa is a highly adaptable Gram-negative opportunistic pathogen, notably due to its large number of transcription regulators. The extracytoplasmic sigma factor (ECFσ) AlgU, responsible for alginate biosynthesis, is also involved in responses to cell wall stress and heat shock via the RpoH alternative σ factor. The SigX ECFσ emerged as a major regulator involved in the envelope stress response via membrane remodeling, virulence and biofilm formation. However, their functional interactions to coordinate the envelope homeostasis in response to environmental variations remain to be determined. The regulation of the putative cmaX-cfrX-cmpX operon located directly upstream sigX was investigated by applying sudden temperature shifts from 37°C. We identified a SigX- and an AlgU- dependent promoter region upstream of cfrX and cmaX, respectively. We show that cmaX expression is increased upon heat shock through an AlgU-dependent but RpoH independent mechanism. In addition, the ECFσ SigX is activated in response to valinomycin, an agent altering the membrane structure, and up-regulates cfrX-cmpX transcription in response to cold shock. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000129044
Veröffentlicht am 27.01.2021
Originalveröffentlichung
DOI: 10.3389/fmicb.2020.579495
Scopus
Zitationen: 2
Web of Science
Zitationen: 2
Dimensions
Zitationen: 2
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 1664-302X
KITopen-ID: 1000129044
HGF-Programm 47.02.06 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IFG
Erschienen in Frontiers in microbiology
Verlag Frontiers Media
Band 11
Seiten Art. Nr.: 579495
Vorab online veröffentlicht am 21.10.2020
Schlagwörter Pseudomonas aeruginosa, temperature, cell wall stress ECF sigma factor, regulation of transcription, membrane fluidity
Nachgewiesen in Scopus
Dimensions
Web of Science
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