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Improving the cathodic biofilm growth capabilities of kyrpidia spormannii EA-1 by undirected mutagenesis

Jung, Tobias; Hackbarth, Max; Horn, Harald; Gescher, Johannes

Abstract:
The biotechnological usage of carbon dioxide has become a relevant aim for future processes. Microbial electrosynthesis is a rather new technique to energize biological CO2 fixation with the advantage to establish a continuous process based on a cathodic biofilm that is supplied with renewable electrical energy as electron and energy source. In this study, the recently characterized cathodic biofilm forming microorganism Kyrpidia spormannii strain EA-1 was used in an adaptive laboratory evolution experiment to enhance its cathodic biofilm growth capabilities. At the end of the experiment, the adapted cathodic population exhibited an up to fourfold higher biofilm accumulation rate, as well as faster substratum coverage and a more uniform biofilm morphology compared to the progenitor strain. Genomic variant analysis revealed a genomically heterogeneous population with genetic variations occurring to various extends throughout the community. Via the conducted analysis we identified possible targets for future genetic engineering with the aim to further optimize cathodic growth. Moreover, the results assist in elucidating the underlying processes that enable cathodic biofilm formation.

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Verlagsausgabe §
DOI: 10.5445/IR/1000129110
Veröffentlicht am 29.01.2021
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Biowissenschaften (IAB)
Institut für Biologische Grenzflächen (IBG)
Engler-Bunte-Institut (EBI)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 2076-2607
KITopen-ID: 1000129110
Erschienen in Microorganisms
Verlag MDPI
Band 9
Heft 77
Seiten 1-15
Schlagwörter microbial electrosynthesis; Kyrpidia spormannii; cathodic biofilm; adaptation; mutagenesis; polyhydroxybutyrate; PHB; bioelectrochemistry
Nachgewiesen in Dimensions
Scopus
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