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A Modular Fluorescent Probe for Viscosity and Polarity Sensing in DNA Hybrid Mesostructures

Ludwanowski, Simon; Samanta, Avik; Loescher, Sebastian; Barner-Kowollik, Christopher 1; Walther, Andreas
1 Institut für Technische Chemie und Polymerchemie (ITCP), Karlsruher Institut für Technologie (KIT)


here exists a critical need in biomedical molecular imaging and diagnostics for molecular sensors that report on slight changes to their local microenvironment with high spatial fidelity. Herein, a modular fluorescent probe, termed StyPy, is rationally designed which features i) an enormous and tunable Stokes shift based on twisted intramolecular charge transfer (TICT) processes with no overlap, a broad emission in the far‐red/near‐infrared (NIR) region of light and extraordinary quantum yields of fluorescence, ii) a modular applicability via facile para‐fluoro‐thiol reaction (PFTR), and iii) a polarity‐ and viscosity‐dependent emission. This renders StyPy as a particularly promising molecular sensor. Based on the thorough characterization on the molecular level, StyPy reports on the viscosity change in all‐DNA microspheres and indicates the hydrophilic and hydrophobic compartments of hybrid DNA‐based mesostructures consisting of latex beads embedded in DNA microspheres. Moreover, the enormous Stokes shift of StyPy enables one to detect multiple fluorophores, while using only a single laser line for excitation in DNA protocells. The authors anticipate that the presented results for multiplexing information are of direct importance for advanced imaging in complex soft matter and biological systems.

Verlagsausgabe §
DOI: 10.5445/IR/1000129379
Veröffentlicht am 05.02.2021
DOI: 10.1002/advs.202003740
Zitationen: 17
Web of Science
Zitationen: 19
Zitationen: 23
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Nanotechnologie (INT)
Institut für Technische Chemie und Polymerchemie (ITCP)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 03.03.2021
Sprache Englisch
Identifikator ISSN: 2198-3844, 2198-3844
KITopen-ID: 1000129379
HGF-Programm 43.21.04 (POF III, LK 01) Molecular Engineering
Erschienen in Advanced science
Verlag Wiley Open Access
Band 8
Heft 5
Seiten Art.-Nr.: 2003740
Vorab online veröffentlicht am 23.12.2020
Schlagwörter DNA mesostructures; molecular probes; protocells; Stokes shift; twisted intramolecular charge transfer; viscosity probes
Nachgewiesen in Dimensions
Web of Science
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