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DGK and DZHK position paper on genome editing: basic science applications and future perspective

Brandes, R. P.; Dueck, A.; Engelhardt, S.; Kaulich, M.; Kupatt, C.; De Angelis, M. T.; Leisegang, M. S.; Noble, Ferdinand le; Moretti, A.; Müller, O. J.; Skryabin, B. V.; Thum, T.; Wurst, W.

Abstract:
For a long time, gene editing had been a scientific concept, which was limited to a few applications. With recent developments, following the discovery of TALEN zinc-finger endonucleases and in particular the CRISPR/Cas system, gene editing has become a technique applicable in most laboratories. The current gain- and loss-of function models in basic science are revolutionary as they allow unbiased screens of unprecedented depth and complexity and rapid development of transgenic animals. Modifications of CRISPR/Cas have been developed to precisely interrogate epigenetic regulation or to visualize DNA complexes. Moreover, gene editing as a clinical treatment option is rapidly developing with first trials on the way. This article reviews the most recent progress in the field, covering expert opinions gathered during joint conferences on genome editing of the German Cardiac Society (DGK) and the German Center for Cardiovascular Research (DZHK). Particularly focusing on the translational aspect and the combination of cellular and animal applications, the authors aim to provide direction for the development of the field and the most frequent applications with their problems.

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Verlagsausgabe §
DOI: 10.5445/IR/1000129526
Veröffentlicht am 10.02.2021
Cover der Publikation
Zugehörige Institution(en) am KIT Zoologisches Institut (ZOO)
Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 0175-9418, 0300-8428, 1435-1803
KITopen-ID: 1000129526
Erschienen in Basic research in cardiology
Verlag Springer
Band 116
Heft 1
Seiten Art.-Nr.: 2
Schlagwörter Animal models; CRISPR/Cas; Genome editing
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Scopus
Web of Science
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