Directed Electron Transfer in Flavin Peptides with Oligoproline‐Type Helical Conformation as Models for Flavin‐Functional Proteins

To mimic the charge separation in functional proteins we studied flavin‐modified peptides as models. They were synthesized as oligoprolines that typically form a polyproline type‐II helix, because this secondary structure supports the electron transfer properties. We placed the flavin as photoexcitable chromophore and electron acceptor at the N‐terminus. Tryptophans were placed as electron donors to direct the electron transfer over 0–3 intervening prolines. Spectroscopic studies revealed competitive photophysical pathways. The reference peptide without tryptophan shows dominant non‐specific ET dynamics, leading to an ion pair formation, whereas peptides with tryptophans have weak non‐specific ET and intensified directed electron transfer. By different excitation wavelengths, we can conclude that the corresponding ion pair state of flavin within the peptide environment has to be energetically located between the S$_{1}$ and S$_{4}$ states, whereas the directed electron transfer to tryptophan occurs directly from the S$_{1}$ state. These photochemical results have fundamental significance for proteins with flavin as redoxactive cofactor.