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Advanced 3D Cell Culture Techniques in Micro-Bioreactors, Part I: A Systematic Analysis of the Literature Published between 2000 and 2020

Grün, Christoph ORCID iD icon 1; Altmann, Brigitte; Gottwald, Eric ORCID iD icon 1
1 Institut für Funktionelle Grenzflächen (IFG), Karlsruher Institut für Technologie (KIT)


Bioreactors have proven useful for a vast amount of applications. Besides classical large-scale bioreactors and fermenters for prokaryotic and eukaryotic organisms, micro-bioreactors, as specialized bioreactor systems, have become an invaluable tool for mammalian 3D cell cultures. In this systematic review we analyze the literature in the field of eukaryotic 3D cell culture in micro-bioreactors within the last 20 years. For this, we define complexity levels with regard to the cellular 3D microenvironment concerning cell–matrix-contact, cell–cell-contact and the number of different cell types present at the same time. Moreover, we examine the data with regard to the micro-bioreactor design including mode of cell stimulation/nutrient supply and materials used for the micro-bioreactors, the corresponding 3D cell culture techniques and the related cellular microenvironment, the cell types and in vitro models used. As a data source we used the National Library of Medicine and analyzed the studies published from 2000 to 2020

Verlagsausgabe §
DOI: 10.5445/IR/1000129666
Veröffentlicht am 12.02.2021
DOI: 10.3390/pr8121656
Zitationen: 7
Web of Science
Zitationen: 7
Zitationen: 8
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 12.2020
Sprache Englisch
Identifikator ISSN: 2227-9717
KITopen-ID: 1000129666
HGF-Programm 47.02.06 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IFG
Erschienen in Processes
Verlag MDPI
Band 8
Heft 12
Seiten Art.-Nr.: 1656
Vorab online veröffentlicht am 15.12.2020
Schlagwörter micro-bioreactor; 3D cell culture; scaffolds; microfluidics; material; cellular microenvironment; tissue engineering; HTS; in vitro models
Nachgewiesen in Dimensions
Web of Science
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