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Siphon-Controlled Automation on a Lab-on-a-Disc Using Event-Triggered Dissolvable Film Valves

Henderson, Brian D.; Kinahan, David J.; Rio, Jeanne; Mishra, Rohit; King, Damien; Torres-Delgado, Sarai M.; Mager, Dario; Korvink, Jan G.; Ducrée, Jens

Abstract:
Within microfluidic technologies, the centrifugal microfluidic "Lab-on-a-Disc" (LoaD) platform offers great potential for use at the PoC and in low-resource settings due to its robustness and the ability to port and miniaturize 'wet bench' laboratory protocols. We present the combination of 'event-triggered dissolvable film valves' with a centrifugo-pneumatic siphon structure to enable control and timing, through changes in disc spin-speed, of the release and incubations of eight samples/reagents/wash buffers. Based on these microfluidic techniques, we integrated and automated a chemiluminescent immunoassay for detection of the CVD risk factor marker C-reactive protein displaying a limit of detection (LOD) of 44.87 ng mL$^{-1}$ and limit of quantitation (LoQ) of 135.87 ng mL$^{-1}$.

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Verlagsausgabe §
DOI: 10.5445/IR/1000130352
Veröffentlicht am 08.03.2021
Originalveröffentlichung
DOI: 10.3390/bios11030073
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Mikrostrukturtechnik (IMT)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 03.2021
Sprache Englisch
Identifikator ISSN: 2079-6374
KITopen-ID: 1000130352
HGF-Programm 43.35.01 (POF IV, LK 01) Platform for Correlative, In Situ & Operando Charakterizat.
Erschienen in Biosensors
Verlag MDPI
Band 11
Heft 3
Seiten Art.-Nr. 73
Vorab online veröffentlicht am 06.03.2021
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