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Acetate as substrate for l-malic acid production with Aspergillus oryzae DSM 1863

Kövilein, Aline 1; Umpfenbach, Julia 1; Ochsenreither, Katrin ORCID iD icon 1
1 Karlsruher Institut für Technologie (KIT)


Microbial malic acid production is currently not able to compete economically with well-established chemical processes using fossil resources. The utilization of inexpensive biomass-based substrates containing acetate could decrease production costs and promote the development of microbial processes. Acetate is a by-product in lignocellulosic hydrolysates and fast pyrolysis products or can be synthesized by acetogens during syngas fermentation. For the fermentation of these substrates, a robust microorganism with a high tolerance for biomass-derived inhibitors is required. Aspergillus oryzae is a suitable candidate due to its high tolerance and broad substrate spectrum. To pave the path towards microbial malic acid production, the potential of acetate as a carbon source for A. oryzae is evaluated in this study.
A broad acetate concentration range was tested both for growth and malic acid production with A. oryzae. Dry biomass concentration was highest for acetic acid concentrations of 40–55 g/L reaching values of about 1.1 g/L within 48 h. Morphological changes were observed depending on the acetate concentration, yielding a pellet-like morphology with low and a filamentous structure with high substrate concentrations. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000130491
Veröffentlicht am 19.03.2021
DOI: 10.1186/s13068-021-01901-5
Zitationen: 9
Web of Science
Zitationen: 7
Zitationen: 11
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Karlsruher Institut für Technologie (KIT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 1754-6834
KITopen-ID: 1000130491
Erschienen in Biotechnology for Biofuels
Verlag Springer Fachmedien Wiesbaden
Band 14
Heft 1
Seiten Art.-Nr.: 48
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
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