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Transcription organizes euchromatin via microphase separation

Hilbert, Lennart; Sato, Yuko; Kuznetsova, Ksenia; Bianucci, Tommaso; Kimura, Hiroshi; Jülicher, Frank; Honigmann, Alf; Zaburdaev, Vasily; Vastenhouw, Nadine L.

Abstract:
In eukaryotes, DNA is packed inside the cell nucleus in the form of chromatin, which consistsof DNA, proteins such as histones, and RNA. Euchromatin, which is permissive for tran-scription, is spatially organized into transcriptionally inactive domains interspersed withpockets of transcriptional activity. While transcription and RNA have been implicated ineuchromatin organization, it remains unclear how their interplay forms and maintains tran-scription pockets. Here we combine theory and experiment to analyze the dynamics ofeuchromatin organization as pluripotent zebrafish cells exit mitosis and begin transcription.We show that accumulation of RNA induces formation of transcription pockets which dis-place transcriptionally inactive chromatin. We propose that the accumulating RNA recruitsRNA-binding proteins that together tend to separate from transcriptionally inactive euchro-matin. Full phase separation is prevented because RNA remains tethered to transcribedeuchromatin through RNA polymerases. Instead, smaller scale microphases emerge that donot grow further and form the typical pattern of euchromatin organization

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Verlagsausgabe §
DOI: 10.5445/IR/1000130758
Veröffentlicht am 19.03.2021
Originalveröffentlichung
DOI: 10.1038/s41467-021-21589-3
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische und Chemische Systeme (IBCS)
Zoologisches Institut (ZOO)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 12.2021
Sprache Englisch
Identifikator ISSN: 2041-1723
KITopen-ID: 1000130758
HGF-Programm 47.14.02 (POF IV, LK 01) Information Storage and Processing in the Cell Nucleus
Erschienen in Nature Communications
Verlag Nature Research
Band 12
Heft 1
Seiten Article no: 1360
Vorab online veröffentlicht am 01.03.2021
Nachgewiesen in Scopus
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