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Comparative Genomics Reveals Prophylactic and Catabolic Capabilities of Actinobacteria within the Fungus-Farming Termite Symbiosis

Murphy, Robert; Benndorf, Rene; Beer, Z. Wilhelm de; Vollmers, John; Kaster, Anne-Kristin; Beemelmanns, Christine; Poulsen, Michael

Abstract:
Actinobacteria, one of the largest bacterial phyla, are ubiquitous in many of Earth’s ecosystems and often act as defensive symbionts with animal hosts. Members of the phylum have repeatedly been isolated from basidiomycete-cultivating fungus-farming termites that maintain a monoculture fungus crop on macerated dead plant substrate. The proclivity for antimicrobial and enzyme production of Actinobacteria make them likely contributors to plant decomposition and defense in the symbiosis. To test this, we analyzed the prophylactic (biosynthetic gene cluster [BGC]) and metabolic (carbohydrate-active enzyme [CAZy]) potential in 16 (10 existing and six new genomes) termite-associated Actinobacteria and compared these to the soil-dwelling close relatives. Using antiSMASH, we identified 435 BGCs, of which 329 (65 unique) were similar to known compound gene clusters, while 106 were putatively novel, suggesting ample prospects for novel compound discovery. BGCs were identified among all major compound categories, including 26 encoding the production of known antimicrobial compounds, which ranged in activity (antibacterial being most prevalent) and modes of action that might suggest broad defensive potential. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000130911
Veröffentlicht am 25.03.2021
Originalveröffentlichung
DOI: 10.1128/mSphere.01233-20
Scopus
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 2379-5042
KITopen-ID: 1000130911
Erschienen in mSphere
Verlag American Society for Microbiology
Band 6
Heft 2
Seiten 1-12
Nachgewiesen in Scopus
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