KIT | KIT-Bibliothek | Impressum | Datenschutz

Regulation of ddb2 expression in blind cavefish and zebrafish reveals plasticity in the control of sunlight-induced DNA damage repair

Zhao, Haiyu; Li, Hongxiang; Du, Juan; Di Mauro, Giuseppe; Lungu-Mitea, Sebastian; Geyer, Nathalie; Vallone, Daniela; Bertolucci, Cristiano; Foulkes, Nicholas S.

Abstract:
We have gained considerable insight into the mechanisms which recognize and repair DNA damage, but how they adapt to extreme environmental challenges remains poorly understood. Cavefish have proven to be fascinating models for exploring the evolution of DNA repair in the complete absence of UV-induced DNA damage and light. We have previously revealed that the Somalian cavefish Phreatichthys andruzzii, lacks photoreactivation repair via the loss of light, UV and ROS-induced photolyase gene transcription mediated by D-box enhancer elements. Here, we explore whether other systems repairing UV-induced DNA damage have been similarly affected in this cavefish model. By performing a comparative study using P. andruzzii and the surface-dwelling zebrafish, we provide evidence for a conservation of sunlight-regulated Nucleotide Excision Repair (NER). Specifically, the expression of the ddb2 gene which encodes a key NER recognition factor is robustly induced following exposure to light, UV and oxidative stress in both species. As in the case of the photolyase genes, D-boxes in the ddb2 promoter are sufficient to induce transcription in zebrafish. ... mehr

Open Access Logo


Verlagsausgabe §
DOI: 10.5445/IR/1000130919
Veröffentlicht am 25.03.2021
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische und Chemische Systeme (IBCS)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 05.02.2021
Sprache Englisch
Identifikator ISSN: 1553-7390, 1553-7404
KITopen-ID: 1000130919
Erschienen in PLoS Genetics
Verlag Public Library of Science (PLoS)
Band 17
Heft 2
Seiten Art.-Nr. e1009356
Nachgewiesen in Web of Science
Scopus
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
KITopen Landing Page