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Chromosome-level reference genome of the soursop (Annona muricata): A new resource for Magnoliid research and tropical pomology

Strijk, Joeri S.; Hinsinger, Damien D.; Roeder, Mareike M. 1; Chatrou, Lars W.; Couvreur, Thomas L. P.; Erkens, Roy H. J.; Sauquet, Hervé; Pirie, Michael D.; Thomas, Daniel C.; Cao, Kunfang
1 Institut für Geographie und Geoökologie (IFGG), Karlsruher Institut für Technologie (KIT)

The flowering plant family Annonaceae includes important commercially grown tropical crops, but development of promising species is hindered by a lack of genomic resources to build breeding programs. Annonaceae are part of the magnoliids, an ancient lineage of angiosperms for which evolutionary relationships with other major clades remain unclear. To provide resources to breeders and evolutionary researchers, we report a chromosome‐level genome assembly of the soursop (Annona muricata). We assembled the genome using 444.32 Gb of DNA sequences (676× sequencing depth) from PacBio and Illumina short‐reads, in combination with 10× Genomics and Bionano data (v1). A total of 949 scaffolds were assembled to a final size of 656.77 Mb, with a scaffold N50 of 3.43 Mb (v1), and then further improved to seven pseudo‐chromosomes using Hi‐C sequencing data (v2; scaffold N50: 93.2 Mb, total size in chromosomes: 639.6 Mb). Heterozygosity was very low (0.06%), while repeat sequences accounted for 54.87% of the genome, and 23,375 protein‐coding genes with an average of 4.79 exons per gene were annotated using de novo, RNA‐seq and homology‐based approaches. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000130938
Veröffentlicht am 26.03.2021
DOI: 10.1111/1755-0998.13353
Zitationen: 5
Zitationen: 7
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Geographie und Geoökologie (IFGG)
Universität Karlsruhe (TH) – Interfakultative Einrichtungen (Interfakultative Einrichtungen)
KIT-Zentrum Klima und Umwelt (ZKU)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 07.2021
Sprache Englisch
Identifikator ISSN: 1755-098X, 1471-8278, 1471-8286, 1755-0998
KITopen-ID: 1000130938
Erschienen in Molecular Ecology Resources
Verlag John Wiley and Sons
Band 21
Heft 5
Seiten 1608-1619
Vorab online veröffentlicht am 11.02.2021
Nachgewiesen in Web of Science
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