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Products released from structurally different dextrans by bacterial and fungal dextranases

Pittrof, Silke L.; Kaufhold, Larissa; Fischer, Anja; Wefers, Daniel

Dextran hydrolysis by dextranases is applied in the sugar industry and the medical sector, but it also has a high potential for use in structural analysis of dextrans. However, dextranases are produced by several organisms and thus differ in their properties. The aim of this study was to comparatively investigate the product patterns obtained from the incubation of linear as well as O3- and O4-branched dextrans with different dextranases. For this purpose, genes encoding for dextranases from Bacteroides thetaiotaomicron and Streptococcus salivarius were cloned and heterologously expressed in Escherichia coli. The two recombinant enzymes as well as two commercial dextranases from Chaetomium sp. and Penicillium sp. were subsequently used to hydrolyze structurally different dextrans. The hydrolysis products were investigated in detail by HPAEC-PAD. For dextranases from Chaetomium sp., Penicillium sp., and Bacteroides thetaiotaomicron, isomaltose was the end product of the hydrolysis from linear dextrans, whereas Penicillium sp. dextranase led to isomaltose and isomaltotetraose. In addition, the latter enzyme also catalyzed a disproportionation reaction when incubated with isomaltotriose. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000131153
Veröffentlicht am 12.04.2021
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Biowissenschaften (IAB)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 2304-8158
KITopen-ID: 1000131153
Erschienen in Foods
Verlag MDPI
Band 10
Heft 2
Seiten 244
Schlagwörter glucans; structure; HPAEC-PAD; enzymatic fingerprinting; oligosaccharides; chromatography; enzymes; exopolysaccharides; enzymatic cleavage
Nachgewiesen in Scopus
Web of Science
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