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Different functional roles of RTR complex factors in DNA repair and meiosis in Arabidopsis and tomato

Whitbread, Amy Leanne; Dorn, Annika; Röhrig, Sarah; Puchta, Holger

Abstract:
The RTR (RecQ/Top3/Rmi1) complex has been elucidated as essential for ensuring genome stability in eukaryotes. Fundamental for the dissolution of Holliday junction (HJ)‐like recombination intermediates, the factors have been shown to play further, partly distinct roles in DNA repair and homologous recombination. Across all kingdoms, disruption of this complex results in characteristic phenotypes including hyper‐recombination and sensitivity to genotoxins. The type IA topoisomerase TOP3α has been shown as essential for viability in various animals. In contrast, in the model plant species Arabidopsis, the top3α mutant is viable. rmi1 mutants are deficient in the repair of DNA damage. Moreover, as opposed to other eukaryotes, TOP3α and RMI1 were found to be indispensable for proper meiotic progression, with mutants showing severe meiotic defects and sterility. We now established mutants of both TOP3α and RMI1 in tomato using CRISPR/Cas technology. Surprisingly, we found phenotypes that differed dramatically from those of Arabidopsis: the top3α mutants proved to be embryo‐lethal, implying an essential role of the topoisomerase in tomato. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000131155
Veröffentlicht am 12.04.2021
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 0960-7412, 1365-313X
KITopen-ID: 1000131155
Erschienen in Plant Journal
Verlag Wiley
Vorab online veröffentlicht am 22.02.2021
Nachgewiesen in Web of Science
Scopus
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