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Morphological characterization of the human corneal epithelium by in vivo confocal laser scanning microscopy

Sterenczak, Katharina A.; Winter, Karsten; Sperlich, Karsten; Stahnke, Thomas; Linke, Stephan; Farrokhi, Sanaz; Klemm, Maren; Allgeier, Stephan; Köhler, Bernd; Reichert, Klaus-Martin; Guthoff, Rudolf F.; Bohn, Sebastian; Stachs, Oliver

Abstract (englisch):
Background: Regarding the growing interest and importance of understanding the cellular changes of the cornea in diseases, a quantitative cellular characterization of the epithelium is becoming increasingly important. Towards this, the latest research offers considerable improvements in imaging of the cornea by confocal laser scanning microscopy (CLSM). This study presents a pipeline to generate normative morphological data of epithelial cell layers of healthy human corneas.
Methods: 3D in vivo CLSM was performed on the eyes of volunteers (n=25) with a Heidelberg Retina Tomograph II equipped with an in-house modified version of the Rostock Cornea Module implementing two dedicated piezo actuators and a concave contact cap. Image data were acquired with nearly isotropic voxel resolution. After image registration, stacks of en-face sections were used to generate full-thickness volume data sets of the epithelium. Beyond that, an image analysis algorithm quantified en-face sections of epithelial cells regarding the depth-dependent mean of cell density, area, diameter, aggregation (Clark and Evans index of aggregation), neighbor count and polygonality.
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Verlagsausgabe §
DOI: 10.5445/IR/1000131628
Veröffentlicht am 20.04.2021
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Automation und angewandte Informatik (IAI)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 05.2021
Sprache Englisch
Identifikator ISSN: 2223-4292, 2223-4306
KITopen-ID: 1000131628
HGF-Programm 43.31.02 (POF IV, LK 01) Devices and Applications
Erschienen in Quantitative imaging in medicine and surgery
Verlag AME Publishing Company (AME)
Band 11
Heft 5
Seiten 1737–1750
Nachgewiesen in Web of Science
Scopus
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