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A modular microfluidic bioreactor to investigate plant cell–cell interactions

Finkbeiner, T. 1; Manz, C. 2; Raorane, M. L. 2; Metzger, C. ORCID iD icon 2; Schmidt-Speicher, L. 1; Shen, N. 1; Ahrens, R. 1; Maisch, J. 2; Nick, P. 2; Guber, A. E. 1
1 Institut für Mikrostrukturtechnik (IMT), Karlsruher Institut für Technologie (KIT)
2 Karlsruher Institut für Technologie (KIT)


Plants produce a wide variety of secondary metabolites, which often are of interest to pharmaceutical and nutraceutical industry. Plant-cell cultures allow producing these metabolites in a standardised manner, independently from various biotic and abiotic factors difficult to control during conventional cultivation. However, plant-cell fermentation proves to be very difficult, since these chemically complex compounds often result from the interaction of different biosynthetic pathways operating in different cell types. To simulate such interactions in cultured cells is a challenge. Here, we present a microfluidic bioreactor for plant-cell cultivation to mimic the cell–cell interactions occurring in real plant tissues. In a modular set-up of several microfluidic bioreactors, different cell types can connect through a flow that transports signals or metabolites from module to module. The fabrication of the chip includes hot embossing of a polycarbonate housing and subsequent integration of a porous membrane and in-plane tube fittings in a two-step ultrasonic welding process. The resulting microfluidic chip is biocompatible and transparent. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000132486
Veröffentlicht am 10.05.2021
DOI: 10.1007/s00709-021-01650-0
Zitationen: 9
Web of Science
Zitationen: 6
Zitationen: 8
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut (BOTANIK)
Institut für Mikrostrukturtechnik (IMT)
Institut für Technik der Informationsverarbeitung (ITIV)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 01.2022
Sprache Englisch
Identifikator ISSN: 0033-183X, 1615-6102
KITopen-ID: 1000132486
HGF-Programm 43.35.02 (POF IV, LK 01) Functionality of Soft Matter and Biomolecular Systems
Erschienen in Protoplasma
Verlag Springer
Band 259
Heft 1
Seiten 173 - 186
Vorab online veröffentlicht am 02.05.2021
Nachgewiesen in Dimensions
Web of Science
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