KIT | KIT-Bibliothek | Impressum | Datenschutz

Geometrically defined environments direct cell division rate and subcellular YAP localization in single mouse embryonic stem cells

Bertels, Sarah 1; Jaggy, Mona 1; Richter, Benjamin 2; Keppler, Stephan 2; Weber, Kerstin 2; Genthner, Elisa 2; Fischer, Andrea C. 3; Thiel, Michael; Wegener, Martin 3,4; Greiner, Alexandra M. 2; Autenrieth, Tatjana J. 1; Bastmeyer, Martin 1
1 Institut für Funktionelle Grenzflächen (IFG), Karlsruher Institut für Technologie (KIT)
2 Karlsruher Institut für Technologie (KIT)
3 Institut für Angewandte Physik (APH), Karlsruher Institut für Technologie (KIT)
4 Institut für Nanotechnologie (INT), Karlsruher Institut für Technologie (KIT)

Abstract:

Mechanotransduction via yes-associated protein (YAP) is a central mechanism for decision-making in mouse embryonic stem cells (mESCs). Nuclear localization of YAP is tightly connected to pluripotency and increases the cell division rate (CDR). How the geometry of the extracellular environment influences mechanotransduction, thereby YAP localization, and decision-making of single isolated mESCs is largely unknown. To investigate this relation, we produced well-defined 2D and 2.5D microenvironments and monitored CDR and subcellular YAP localization in single mESCs hence excluding cell–cell interactions. By systematically varying size and shape of the 2D and 2.5D substrates we observed that the geometry of the growth environment affects the CDR. Whereas CDR increases with increasing adhesive area in 2D, CDR is highest in small 2.5D micro-wells. Here, mESCs attach to all four walls and exhibit a cross-shaped cell and nuclear morphology. This observation indicates that changes in cell shape are linked to a high CDR. Inhibition of actomyosin activity abrogate these effects. Correspondingly, nuclear YAP localization decreases in inhibitor treated cells, suggesting a relation between cell shape, intracellular forces, and cell division rate. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000133163
Veröffentlicht am 22.05.2021
Originalveröffentlichung
DOI: 10.1038/s41598-021-88336-y
Scopus
Zitationen: 11
Web of Science
Zitationen: 8
Dimensions
Zitationen: 11
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Physik (APH)
Institut für Funktionelle Grenzflächen (IFG)
Institut für Nanotechnologie (INT)
Universität Karlsruhe (TH) – Interfakultative Einrichtungen (Interfakultative Einrichtungen)
Karlsruhe School of Optics & Photonics (KSOP)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 2045-2322
KITopen-ID: 1000133163
HGF-Programm 43.32.02 (POF IV, LK 01) Designed Optical Materials
Erschienen in Scientific Reports
Verlag Nature Research
Band 11
Heft 1
Seiten Art.-Nr.: 9269
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Nachgewiesen in Dimensions
Scopus
Web of Science
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
KITopen Landing Page