Root Digger: a root placement program for phylogenetic trees

Background
In phylogenetic analysis, it is common to infer unrooted trees. However, knowing the root location is desirable for downstream analyses and interpretation. There exist several methods to recover a root, such as molecular clock analysis (including midpoint rooting) or rooting the tree using an outgroup. Non-reversible Markov models can also be used to compute the likelihood of a potential root position.

Results
We present a software called RootDigger which uses a non-reversible Markov model to compute the most likely root location on a given tree and to infer a confidence value for each possible root placement. We find that RootDigger is successful at finding roots when compared to similar tools such as IQ-TREE and MAD, and will occasionally outperform them. Additionally, we find that the exhaustive mode of RootDigger is useful in quantifying and explaining uncertainty in rooting positions.

Conclusions
RootDigger can be used on an existing phylogeny to find a root, or to asses the uncertainty of the root placement