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Performance of Different Diagnostic PD-L1 Clones in Head and Neck Squamous Cell Carcinoma

Ribbat-Idel, J.; Dressler, F. F.; Krupar, R.; Watermann, C.; Paulsen, F.-O.; Kuppler, P.; Klapper, L.; Offermann, A.; Wollenberg, B.; Rades, D.; Laban, S.; Reischl, M. 1; Bruchhage, K.-L.; Idel, C.; Perner, S.
1 Karlsruher Institut für Technologie (KIT)

Abstract:
Background: The approval of immune checkpoint inhibitors in combination with specific diagnostic biomarkers presents new challenges to pathologists as tumor tissue needs to be tested for expression of programmed death-ligand 1 (PD-L1) for a variety of indications. As there is currently no requirement to use companion diagnostic assays for PD-L1 testing in Germany different clones are used in daily routine. While the correlation of staining results has been tested in various entities, there is no data for head and neck squamous cell carcinomas (HNSCC) so far.

Methods: We tested five different PD-L1 clones (SP263, SP142, E1L3N, 22-8, 22C3) on primary HNSCC tumor tissue of 75 patients in the form of tissue microarrays. Stainings of both immune and tumor cells were then assessed and quantified by pathologists to simulate real-world routine diagnostics. The results were analyzed descriptively and the resulting staining pattern across patients was further investigated by principal component analysis and non-negative matrix factorization clustering.

Results: Percentages of positive immune and tumor cells varied greatly. Both the resulting combined positive score as well as the eligibility for certain checkpoint inhibitor regimens was therefore strongly dependent on the choice of the antibody. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000133574
Veröffentlicht am 03.06.2021
Originalveröffentlichung
DOI: 10.3389/fmed.2021.640515
Scopus
Zitationen: 4
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Zitationen: 6
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Automation und angewandte Informatik (IAI)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 2296-858X
KITopen-ID: 1000133574
HGF-Programm 47.14.02 (POF IV, LK 01) Information Storage and Processing in the Cell Nucleus
Erschienen in Frontiers in Medicine
Verlag Frontiers Media
Band 8
Seiten Art.-Nr.: 640515
Vorab online veröffentlicht am 27.04.2021
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