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Carbon monoxide induced metabolic shift in the carboxydotrophic parageobacillus thermoglucosidasius dsm 6285

Aliyu, Habibu 1; Kastner, Ronnie 1; Maayer, Pieter de; Neumann, Anke 1
1 Institut für Bio- und Lebensmitteltechnik (BLT), Karlsruher Institut für Technologie (KIT)

Abstract:
Parageobacillus thermoglucosidasius is known to catalyse the biological water gas shift (WGS) reaction, a pathway that serves as a source of alternative energy and carbon to a wide variety of bacteria. Despite increasing interest in this bacterium due to its ability to produce biological hydrogen through carbon monoxide (CO) oxidation, there are no data on the effect of toxic CO gas on its physiology. Due to its general requirement of O$_{2}$, the organism is often grown aerobically to generate biomass. Here, we show that carbon monoxide (CO) induces metabolic changes linked to distortion of redox balance, evidenced by increased accumulation of organic acids such as acetate and lactate. This suggests that P. thermoglucosidasius survives by expressing several alternative pathways, including conversion of pyruvate to lactate, which balances reducing equivalents (oxidation of NADH to NAD+), and acetyl-CoA to acetate, which directly generates energy, while CO is binding terminal oxidases. The data also revealed clearly that P. thermoglucosidasius gained energy and grew during the WGS reaction. Combined, the data provide critical information essential for further development of the biotechnological potential of P. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000133652
Veröffentlicht am 08.06.2021
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 2076-2607
KITopen-ID: 1000133652
Erschienen in Microorganisms
Verlag MDPI
Band 9
Heft 5
Seiten 1090
Schlagwörter acetate; biological water gas shift reaction; carbon monoxide; lactate; Parageobacillus thermoglucosidasius; reducing equivalents
Nachgewiesen in Scopus
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