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DNA-Directed Assembly of a Cell-Responsive Biohybrid Interface for Cargo Release

Sun, Pengchao 1; Scharnweber, Tim 1; Wadhwani, Parvesh 1; Rabe, Kersten S. 1; Niemeyer, Christof M. 1
1 Institut für Biologische Grenzflächen (IBG), Karlsruher Institut für Technologie (KIT)

The development of a DNA-based cell-responsive biohybrid interface that can be used for spatially confined release of molecular cargo is reported. To this end, tailored DNA–protein conjugates are designed as gatekeepers that can be specifically cleaved by matrix metalloproteases (MMPs), which are secreted by many cancer cells. These gatekeepers can be installed by DNA hybridization on the surface of mesoporous silica nanoparticles (MSNs). The MSNs display another orthogonal DNA oligonucleotide that can be exploited for site-selective immobilization on solid glass surfaces to yield micropatterned substrates for cell adhesion. Using the human fibrosarcoma cell line HT1080 that secretes MMPs, it is demonstrated that the biohybrid surface is specifically modified by the cells to release both MSN-bound gatekeeper proteins and the encapsulated cargo peptide KLA. In view of the enormously high modularity of the system presented here, this approach promising for applications in drug delivery, tissue engineering, or other areas of nanobiotechnology is considered.

Verlagsausgabe §
DOI: 10.5445/IR/1000133659
Veröffentlicht am 09.06.2021
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 15.07.2021
Sprache Englisch
Identifikator ISSN: 2366-9608
KITopen-ID: 1000133659
HGF-Programm 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems
Weitere HGF-Programme 47.14.01 (POF IV, LK 01) Molekular Information Processing in Cellular Systems
Erschienen in Small Methods
Verlag Wiley-VCH Verlag
Band 5
Heft 7
Seiten Art-Nr. 2001049
Vorab online veröffentlicht am 24.05.2021
Nachgewiesen in Web of Science
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